Scanning electron microscopy of thermoplasma acidophilum. While triosephosphate isomerase tpi, a wellknown glycolytic enzyme, is not involved in the ed pathway in t. Download fulltext pdf download fulltext pdf the intein of the thermoplasma aatpase a subunit. Thermoplasma definition of thermoplasma by medical dictionary. Thermoplasma, genus thermoplasma, any of a group of prokaryotic organisms organisms whose cells lack a defined nucleus in the domain archaea that are noted for their ability to thrive in hot, acidic environments. Purification and characterization of geranylgeranylglyceryl. The chaperones of the archaeon thermoplasma acidophilum.
Species of the genus thermoplasma do not possess a rigid cell wall, but are only delimited by a plasma membrane. Thermoplasma is a thermophile, meaning that it grows best in hot environments, usually between 55 and 60 degrees celsius. Structure of thermoplasmaquinone from thermoplasma acidophilum. Species of thermoplasma are facultative aerobes, growing either aerobically or anaerobically by sulfur respiration. Full text get a printable copy pdf file of the complete article 1. Lupas,3 department of molecular structural biology, maxplanckinstitute for biochemistry, am klopferspitz 18a, d82152 martinsried, germany. Another conserved cys residue is highlighted in green. Thermoplasma langworthy major reference works wiley. Crystal structure of mevalonate3kinase from thermoplasma acidophilum mevalonate bound.
The temperature optimum is around 90c and the ph optimum 89 8. Sep 28, 2000 thermoplasma acidophilum is a thermoacidophilic archaeon that thrives at 59 degrees c and ph 2, which was isolated from selfheating coal refuse piles and solfatara fields. This article is within the scope of wikiproject microbiology, a collaborative effort to improve the coverage of microbiology on wikipedia. From a morphological point of view thermoplasma resembles the mycoplasmas in that it lacks a. Download fulltext pdf a histonelike protein hta from thermoplasma acidophilum article pdf available in journal of biological chemistry 2562. Thermoplasma acidophilum is a thermophilic heterotrophic prokaryote and is among the most acidophilic organism known. Histones are a principal constituent of chromatin in eukaryotes and fundamental to our understanding of eukaryotic gene regulation. In taxonomy, thermoplasma is a genus of the thermoplasmataceae thermoplasma is a genus of archaea. The dnabinding protein hta from thermoplasma acidophilum is. The glycerol glycolipids and phospholipids of thermoplasma appeared to.
In addition to this splicing activity, most reported inteins also contain an endonuclease domain that is important in intein propagation. The structure of the methylsubstituted menaquinone designated thermoplasmaquinone from the extremely thermophilic and acidophilic. Morphological variation of new thermoplasma acidophilum isolates. Biochemical characterization of the minichromosome. From microbial communities to cells internet archive. Thermoplasma volcanium is a moderate thermoacidophilic archaea isolated from acidic hydrothermal vents and solfatara fields. Engineered thermoplasma acidophilum factor f3 mimics human. The genome sequence of the thermoacidophilic scavenger. Pdf we isolated 12 strains of thermoplasma acidophilum from hot springs in hakone, japan.
Acidophily in thermoplasma can be related to a significant reduction in repulsing negative charges on the membrane proteins. Perhaps the organism we have isolated which has elicited the most interest and some controversy has been thermoplasma. Characterization of thermoplasma species cultured from sampling on tangkuban perahu, indonesia. Get a printable copy pdf file of the complete article 1. The genus name is derived from the greek therme and plasma, meaning warmth or heat and formative substance, respectively, which describe the thermophilic heat. Characterization of the membranes of thermoplasma acidophilum. Thermoplasma acidophilum is an archaeon, the type species of its genus. Morphological variation of new thermoplasma acidophilum isolates from japanese hot springs article pdf available in applied and environmental microbiology 619. The chaperones of the archaeon thermoplasma acidophilum andreas ruepp,1 beate rockel,2 irina gutsche, wolfgang baumeister, and andrei n. It was found and first isolated from selfheating coal refuse piles and solfatara fields. A gene from the thermophilic archaeon thermoplasma volcanium encoding an lthreonine dehydrogenase lthrdh with a predicted amino acid sequence that was remarkably similar to the sequence of udpgalactose 4epimerase gale was overexpressed in escherichia coli, and its product was purified and characterized. Thermoplasma acidophilum is a thermoacidophilic archaeon. Pdf morphological variation of new thermoplasma acidophilum.
Effects of temperature on leucylpnitroanilide hydrolysis by factor f3 mutant. Links to pubmed are also available for selected references. We purified a geranylgeranylglyceryl phosphate gggp synthase from thermoplasma acidophilum by several steps of chromatography. Pdf characterization of thermoplasma species cultured. Based on the proteinasefragmentmasspattern analysis of the sdspage band of the partially purified protein, the dna sequence encoding the protein was identified from the whole genome sequence database of the species. Results the gene encoding the thermoplasma acidophilum aatpase catalytic subunit a is. If you would like to participate, please visit the project page, where you can join the discussion and see a list of open tasks. Thermoplasma was first isolated in the 1960s, in a coal pile in indiana. The substrate and the enzyme were preincubated at 40, 50, 60, 65, 70, 75, 80 and 90 c for 30 min and measured at 405 nm. Download mendeley download endnote solution structure of 30s ribosomal protein s27a from thermoplasma acidophilumnortheast structural genomics consortium target tat88ontario center for structural proteomics target ta1093. Thermoplasma acidophilum thermoplasma volcanium thermoplasma sp.
Pdf characterization of thermoplasma species cultured from. In taxonomy, the thermoplasmatales are an order of the thermoplasmata. Thermoplasma are facultative anaerobes and respire using sulfur and organic carbon. Among the organisms tested here, the hydrogenation activity was detected only in the thermoacidophilic archaeon thermoplasma acidophilum, whose core lipids are composed of archaeol and caldarchaeol containing a 08 cyclopentane ring. Twentythree isolates of motile, cell walldeficient, thermoacidophilic archaebacteria were obtained from hot acidic springs, soils and sediments within solfatara. Thermoplasma acidophilum darland, brock, samsonoff and conti 1970, 1418 thermoplasma.
Presentation mode open print download current view. In taxonomy, thermoplasma is a genus of the thermoplasmataceae. They do not contain a cell wall but instead contain a unique membrane composed mainly of a tetraether lipoglycan. This genus is most famous for its acidophilia, preferring ph range of 0. Full text full text is available as a scanned copy of the original print version. Pdf dolichylphosphomannose synthase from the archae. Citrate synthase from the thermophilic archaebacteria.
Multiple sequence alignment of type i and ii frmsrs. Structural and biochemical analysis of a type ii free. The thermoplasma proteasome has chymotryptic specificity when assayed with fluorogenic peptides. Furthermore, it acts as the base for the cleavage of the acylester intermediate, thereby releasing thr1 o.
It belongs to the thermoplasmata, which thrive in acidic and hightemperature environments. Localization of subunits in proteasomes from thermoplasma. A genus of bacteria order mycoplasmatales that possesses the same characteristics as the organisms in the genus mycoplasma except that the thermoplasmas do not require sterol for growth, have an optimal temperature of 5559c, have an optimal ph of 1. Morphological variation of new thermoplasma acidophilum isolates from japanese hot springs. The genus name is derived from the greek therme and plasma, meaning warmth or. The main phospholipid mpl of thermoplasma acidophilum dsm 1728 was isolated, purified and physicochemically. Hu has been investigated extensively for its role in bacterial genome organization and gene regulation. Pdf the intein of the thermoplasma aatpase a subunit. Article pdf available in applied and environmental microbiology 619. Inteins are selfish genetic elements that excise themselves from the host protein during post translational processing, and religate the host protein with a peptide bond. The catalytic cys residues in type i and ii frmsr enzymes are highlighted in red and the resolving cys in type i frmsr enzymes are highlighted in blue.
We isolated 12 strains of thermoplasma acidophilum from hot springs in hakone, japan. The gene encoding the thermoplasma acidophilum aatpase catalytic subunit a is the only. Picrophilus is currently the most acidophilic of all known organisms, being capable of growing at a ph of 0. Pdf a histonelike protein hta from thermoplasma acidophilum. Download fulltext pdf dolichylphosphomannose synthase from the archae thermoplasma acidophilum article pdf available in glycobiology 68. Nov 11, 2019 this manuscript focuses on the hta protein of thermoplasma acidophilum, a hyperthermophilic archaeal species. Thermoplasma acidophilum is a thermophilic archaeon that uses both nonphosphorylative entnerdoudoroff ed pathway and embdenmeyerhofparnas emp pathway for glucose degradation. Thermoplasma is a chemoorganotroph and grows optimally at 55c and ph 2 in complex media. Activity assays are generally performed at 60c, ph 7. Thermoplasma acidophilum and thermoplasma volcanium sp. It is highly flagellated and grows optimally at 56 c 3 f and ph 1. All are acidophiles, growing optimally at ph below 2.
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